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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METTL2 Polyclonal antibody, PBS Only

METTL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16983-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL2 Polyclonal antibody (16983-1-AP)

    WB analysis using 16983-1-AP (same clone as 16983-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16983-1-AP (METTL2B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using METTL2 Polyclonal antibody (16983-1-AP)

    IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using METTL2 Polyclonal antibody (16983-1-AP)

    IHC staining of mouse colon using 16983-1-AP (same clone as 16983-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16983-1-AP (METTL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using METTL2 Polyclonal antibody (16983-1-AP)

    IF Staining of MCF-7 using 16983-1-AP (same clone as 16983-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL2 antibody (16983-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16983-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16983-1-PBS targets METTL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL2 fusion protein Ag10410 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 2B
Calculated molecular weight 313 aa, 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC107586
Gene symbol METTL2B
Gene ID (NCBI) 55798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.