METTL2 Recombinant antibody
METTL2 Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
241490A3
Cat no : 84257-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, PC-3 cells |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 84257-1-RR is METTL2 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | METTL2 fusion protein Ag10410 相同性解析による交差性が予測される生物種 |
Full Name | methyltransferase like 2B |
Calculated molecular weight | 313 aa, 36 kDa |
Observed molecular weight | 36~43 kDa |
GenBank accession number | BC107586 |
Gene symbol | METTL2B |
Gene ID (NCBI) | 55798 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.
Protocols
Product Specific Protocols | |
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WB protocol for METTL2 antibody 84257-1-RR | Download protocol |
IF protocol for METTL2 antibody 84257-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |