METTL2 Recombinant antibody

METTL2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241490A3

Cat no : 84257-1-RR

Synonyms

METTL2B, METTL2A/B, METTL2A, Methyltransferase-like protein 2B, methyltransferase like 2B


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Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells, PC-3 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84257-1-RR is METTL2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL2 fusion protein Ag10410 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 2B
Calculated molecular weight 313 aa, 36 kDa
Observed molecular weight36~43 kDa
GenBank accession numberBC107586
Gene symbol METTL2B
Gene ID (NCBI) 55798
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL2A and METTL2B are required for the threonyl and argininyl tRNA methylation. METTL2 methyltransferase is responsible for 3-methylcytosine modification of arginine tRNAs in mammals. METTL2A and METTL2B are in higher homology 99%. This antibody detects both METTL2A and METTL2B.

Protocols

Product Specific Protocols
WB protocol for METTL2 antibody 84257-1-RRDownload protocol
IF protocol for METTL2 antibody 84257-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols