Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis of HeLa using 15073-1-AP
WB result of METTL3 antibody (15073-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL3 transfected HeLa cells.
WB analysis using 15073-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 15073-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 15073-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 15073-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15073-1-AP (METTL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15073-1-AP
IP result of anti-METTL3 (IP:15073-1-AP, 4ug; Detection:15073-1-AP 1:4000) with HEK-293 cells lysate 1800 ug.
IHC staining of human renal cell carcinoma using 15073-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15073-1-AP (METTL3 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15073-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15073-1-AP (METTL3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 15073-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 15073-1-AP (METTL3 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 15073-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15073-1-AP (METTL3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 15073-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using METTL3 antibody (15073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse testis using 15073-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using METTL3 antibody (15073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 15073-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using METTL3 antibody (15073-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Featured Product
KD/KO Validated

METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 15073-1-AP

Publications(523)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, ELISA

Synonyms

EC:2.1.1.348, hMETTL3, Methyltransferase-like protein 3, MT A70, MTA70

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (523)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HT-1080 cells, mouse testis tissue, HepG2 cells, Jurkat cells, Neuro-2a cells, A549 cells, NCCIT cells, COS-7 cells, rat testis tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human renal cell carcinoma tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse testis tissue
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:750-1:3000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 184 publications below
WB	See 469 publications below
IHC	See 80 publications below
IF	See 59 publications below
IP	See 18 publications below
CoIP	See 13 publications below
ChIP	See 4 publications below
RIP	See 29 publications below

Product Information

15073-1-AP targets METTL3 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, zebrafish, hamster, goat, drosophila
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7110 Product name: Recombinant human METTL3 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 229-580 aa of BC001650 Sequence: LNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRSRGRAQVQEFCDYGTKEECMKASDADRPCRKLHFRRIINKHTDESLGDCSFLNTCFHMDTCKYVHYEIDACMDSEAPGSKDHTPSQELALTQSVGGDSSADRLFPPQWICCDIRYLDVSILGKFAVVMADPPWDIHMELPYGTLTDDEMRRLNIPVLQDDGFLFLWVTGRAMELGRECLNLWGYERVDEIIWVKTNQLQRIIRTGRTGHWLNHGKEHCLVGVKGNPQGFNQGLDCDVIVAEVRSTSHKPDEIYGMIERLSPGTRKIELFGRPHNVQPNWITLGNQLDGIHLLDPDVVARFKQRYPDGIISKPKNL 相同性解析による交差性が予測される生物種
Full Name	methyltransferase like 3
Calculated molecular weight	64 kDa
Observed molecular weight	65-70 kDa
GenBank accession number	BC001650
Gene Symbol	METTL3
Gene ID (NCBI)	56339
RRID	AB_2142033
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86U44
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
IF protocol for METTL3 antibody 15073-1-AP	Download protocol
IHC protocol for METTL3 antibody 15073-1-AP	Download protocol
IP protocol for METTL3 antibody 15073-1-AP	Download protocol
WB protocol for METTL3 antibody 15073-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity. Authors - Jiadong Liu KO Validated View Article
mouse	WB	Nature METTL3 regulates heterochromatin in mouse embryonic stem cells. Authors - Wenqi Xu KO Validated View Article
human	WB	Nature mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis. Authors - Junho Choe KD Validated View Article
human	WB	Nature N(6)-methyladenosine-dependent regulation of messenger RNA stability. Authors - Wang Xiao X KD Validated View Article
human	WB	Mol Cancer A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis Authors - Kaixuan Zeng KD Validated View Article
mouse	WB,IF	Science Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Authors - Shay Geula KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 15073-1-AP

WB analysis using 15073-1-AP

WB analysis using 15073-1-AP

WB analysis using 15073-1-AP

WB analysis using 15073-1-AP

IP experiment of HEK-293 using 15073-1-AP

IHC staining of human renal cell carcinoma using 15073-1-AP

IHC staining of human lung cancer using 15073-1-AP

IHC staining of human renal cell carcinoma using 15073-1-AP

IHC staining of human lung cancer using 15073-1-AP

IF Staining of mouse testis using 15073-1-AP

IF Staining of mouse testis using 15073-1-AP

IF Staining of MCF-7 using 15073-1-AP

METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The RNA m6A reader YTHDC1 silences retrotransposons and guards ES cell identity.

METTL3 regulates heterochromatin in mouse embryonic stem cells.

mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis.

N(6)-methyladenosine-dependent regulation of messenger RNA stability.

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Stem cells. m6A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation.