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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METTL3 Polyclonal antibody

METTL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29314-1-AP

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL3 Polyclonal antibody (29314-1-AP)

    WB analysis using 29314-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using METTL3 Polyclonal antibody (29314-1-AP)

    WB analysis using 29314-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29314-1-AP (METTL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL3 Polyclonal antibody (29314-1-AP)

    IHC staining of human lung cancer using 29314-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using METTL3 Polyclonal antibody (29314-1-AP)

    IHC staining of human lung cancer using 29314-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using METTL3 Polyclonal antibody (29314-1-AP)

    IHC staining of human stomach cancer using 29314-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using METTL3 Polyclonal antibody (29314-1-AP)

    IHC staining of human stomach cancer using 29314-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 29314-1-AP (METTL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Neuro-2a cells, K-562 cells, MCF-7 cells, COS-7 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29314-1-AP targets METTL3 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Monkey samples.

Tested Reactivity Human, Mouse, Rat, Monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL3 fusion protein Ag30638 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC001650
Gene symbol METTL3
Gene ID (NCBI) 56339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is a component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL3 antibody 29314-1-APDownload protocol
IHC protocol for METTL3 antibody 29314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols