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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METTL4 Polyclonal antibody

METTL4 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17718-1-AP

Synonyms

HsT661, methyltransferase like 4, METTL4

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human skin tissue using METTL4 Polyclonal antibody (17718-1-AP)

    IHC staining of human skin using 17718-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17718-1-AP (METTL4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using METTL4 Polyclonal antibody (17718-1-AP)

    IHC staining of human skin using 17718-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 17718-1-AP (METTL4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using METTL4 Polyclonal antibody (17718-1-AP)

    IF Staining of HeLa using 17718-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17718-1-AP (METTL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

17718-1-AP targets METTL4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL4 fusion protein Ag12093 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 4
Calculated molecular weight 472 aa, 54 kDa
GenBank accession numberBC111020
Gene symbol METTL4
Gene ID (NCBI) 64863
RRIDAB_2142047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methyltransferase like protein 4 (METTL4) is a internal m6Am methyltransferase, which mediates N6-methylation of Am30 on U2 snRNA in the context of an AAG motif in vivo and in vitro. METTL4 belongs to the MT-A70-like family, and like its paralogs METTL3/14, METTL4 is conserved from yeast to human.

Protocols

Product Specific Protocols
IHC protocol for METTL4 antibody 17718-1-APDownload protocol
IF protocol for METTL4 antibody 17718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

EMBO J

PGE2 -EP3 axis promotes brown adipose tissue formation through stabilization of WTAP RNA methyltransferase.

Authors - Xixi Tao
View Article
humanWB

Am J Cancer Res

Binding of RNA m6A by IGF2BP3 triggers chemoresistance of HCT8 cells via upregulation of ABCB1.

Authors - Zhongmin Yang
View Article