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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METTL6 Recombinant antibody, PBS Only

METTL6 Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241482F12

Cat no : 84209-4-PBS

Synonyms

tRNA N(3)-methylcytidine methyltransferase METTL6, Methyltransferase-like protein 6, methyltransferase like 6, hMETTL6, EC:2.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL6 Recombinant antibody (84209-4-RR)

    WB analysis using 84209-4-RR (same clone as 84209-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84209-4-RR (METTL6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84209-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84209-4-RR

    Affinity and Kinetic Characterization of 84209-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84209-4-RR against Human METTL6 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 84209-4-PBS is METTL6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL6 fusion protein Ag9714 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 6
Calculated molecular weight 255 aa, 30 kDa
Observed molecular weight 30-32 kDa
GenBank accession numberBC022400
Gene symbol METTL6
Gene ID (NCBI) 131965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent non-tumor tissues. CRISPR/Cas9 mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).