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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

METTL6 Recombinant antibody

METTL6 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241482F9

Cat no : 84209-5-RR

Synonyms

tRNA N(3)-methylcytidine methyltransferase METTL6, Methyltransferase-like protein 6, methyltransferase like 6, hMETTL6, EC:2.1.1.-

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using METTL6 Recombinant antibody (84209-5-RR)

    IF Staining of HeLa using 84209-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using METTL6 antibody (84209-5-RR, Clone: 241482F9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84209-5-RR

    Affinity and Kinetic Characterization of 84209-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84209-5-RR against Human METTL6 were performed. The affinity constant is 7.69 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84209-5-RR is METTL6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen METTL6 fusion protein Ag9714 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 6
Calculated molecular weight 255 aa, 30 kDa
GenBank accession numberBC022400
Gene symbol METTL6
Gene ID (NCBI) 131965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methyltransferase-like protein 6 (METTL6) belongs to the METL family. METTL6 is as a crucial regulator of tumor cell growth, and METTL6 is a bona fide transfer RNA (tRNA) methyltransferase, catalyzing the formation of 3-methylcytidine at C32 of specific serine tRNA isoacceptors (PMID:32923617). METTL6 is a member of the RNA methyltransferase family that has been identified in many cancers. The mRNA expression levels of METTL6 were significantly upregulated in HCC tumor tissues compared to that in adjacent nontumor tissues. CRISPR/Cas9mediated knockout of METTL6 in hepatocellular carcinoma cell lines remarkably inhibited colony formation, cell proliferation, cell migration, cell invasion and cell attachment ability (PMID:34913069).

Protocols

Product Specific Protocols
IF protocol for METTL6 antibody 84209-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols