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  • Featured Product
  • KD/KO Validated

METTL7A Polyclonal antibody

METTL7A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17092-1-AP

Synonyms

AAM B, methyltransferase like 7A, METTL7A, Protein AAM B, UNQ1902/PRO4348

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using METTL7A Polyclonal antibody (17092-1-AP)

    WB analysis using 17092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using METTL7A Polyclonal antibody (17092-1-AP)

    WB analysis using 17092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17092-1-AP (METTL7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using METTL7A Polyclonal antibody (17092-1-AP)

    WB analysis of HepG2 using 17092-1-AP

    WB result of METTL7A antibody (17092-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-METTL7A transfected HepG2 cells.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human liver cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using METTL7A Polyclonal antibody (17092-1-AP)

    IHC staining of human thyroid cancer using 17092-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 17092-1-AP (METTL7A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, mouse lung tissue, rat lung tissue, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17092-1-AP targets METTL7A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen METTL7A fusion protein Ag10013 相同性解析による交差性が予測される生物種
Full Name methyltransferase like 7A
Calculated molecular weight 28 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC008180
Gene symbol METTL7A
Gene ID (NCBI) 25840
RRIDAB_2878348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for METTL7A antibody 17092-1-APDownload protocol
IHC protocol for METTL7A antibody 17092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols