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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    WB analysis of human kidney using 17661-1-AP (same clone as 17661-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    WB analysis of mouse heart using 17661-1-AP (same clone as 17661-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    WB analysis of mouse lung using 17661-1-AP (same clone as 17661-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat lung tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    WB analysis of rat lung using 17661-1-AP (same clone as 17661-1-PBS)

    rat lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human ovary using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human ovary using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human placenta using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human placenta using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human testis using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human testis using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human skin using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human skin using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human spleen using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human spleen using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using MFAP4 Polyclonal antibody (17661-1-AP)

    IHC staining of human lung using 17661-1-AP (same clone as 17661-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17661-1-PBS in a different storage buffer formulation.

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MFAP4 Polyclonal antibody, PBS Only

MFAP4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17661-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17661-1-PBS targets MFAP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFAP4 fusion protein Ag11920 相同性解析による交差性が予測される生物種
Full Name microfibrillar-associated protein 4
Calculated molecular weight 255 aa, 29 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC062415
Gene Symbol MFAP4
Gene ID (NCBI) 4239
RRIDAB_2142355
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55083
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)