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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MFG-E8 Monoclonal antibody

MFG-E8 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A9

Cat no : 67797-1-Ig

Synonyms

BA46, Breast epithelial antigen BA46, EDIL1, HMFG, hP47, HsT19888, Lactadherin, MFG E8, MFGE8, MFG-E8, MFGM, Milk fat globule EGF factor 8, OAcGD3S, SED1, SPAG10

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human milk using MFG-E8 Monoclonal antibody (67797-1-Ig)

    WB analysis of human milk using 67797-1-Ig

    human milk were subjected to SDS PAGE followed by western blot with 67797-1-Ig (MFG-E8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MFG-E8 Monoclonal antibody (67797-1-Ig)

    WB analysis of human placenta using 67797-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67797-1-Ig (MFG-E8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MFG-E8 Monoclonal antibody (67797-1-Ig)

    IHC staining of human placenta using 67797-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MFG-E8 Monoclonal antibody (67797-1-Ig)

    IHC staining of human placenta using 67797-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67797-1-Ig (MFG-E8 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inhuman milk, human placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:4000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67797-1-Ig targets MFG-E8 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MFG-E8 fusion protein Ag30666 相同性解析による交差性が予測される生物種
Full Name milk fat globule-EGF factor 8 protein
Calculated molecular weight 43 kDa
Observed molecular weight43 kDa
GenBank accession numberBC003610
Gene symbol MFGE8
Gene ID (NCBI) 4240
RRIDAB_2918561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Milk fat globule-epidermal growth factor-factor 8 (MFG-E8), also known as lactadherin, is a multifunctional secreted glycoprotein exhibiting versatile functions in cell physiology affecting health and diseases. It plays roles in clearance of apoptotic cells, anti-inflammatory, wound healing, arterial remodeling, angiogenesis, enhancing tumorigenicity and cancer metastasis (PMID: 23972256). MFG-E8 consists of two-repeated EGF-likedomains, a mucin-like domain and two-repeated discoidin-like domains (C-domains), and contains an integrin-binding motif (RGD sequence) in the EGF-like domain (PMID: 11856354). It shows ubiquitous pattern of expression in various types of cells and tissues. Altered expression of MFG-E8 causes disruptions of homeostasis and is correlated with a number of diseases (PMID: 27429513).

Protocols

Product Specific Protocols
WB protocol for MFG-E8 antibody 67797-1-IgDownload protocol
IHC protocol for MFG-E8 antibody 67797-1-IgDownload protocol
FC protocol for MFG-E8 antibody 67797-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols