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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MFN1 Monoclonal antibody (66776-1-Ig)

    WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MFN1 Monoclonal antibody (66776-1-Ig)

    WB analysis using 66776-1-Ig (same clone as 66776-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66776-1-Ig (MFN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MFN1 Monoclonal antibody (66776-1-Ig)

    IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MFN1 Monoclonal antibody (66776-1-Ig)

    IHC staining of human prostate cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MFN1 Monoclonal antibody (66776-1-Ig)

    IHC staining of human colon cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MFN1 Monoclonal antibody (66776-1-Ig)

    IHC staining of human colon cancer using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66776-1-Ig (MFN1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MFN1 Monoclonal antibody (66776-1-Ig)

    IF Staining of HepG2 using 66776-1-Ig (same clone as 66776-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MFN1 antibody (66776-1-Ig, Clone: 3F11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66776-1-PBS in a different storage buffer formulation.

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MFN1 Monoclonal antibody, PBS Only

MFN1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66776-1-PBS
Clone No.3F11C11

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp762F247, FLJ20693, Fzo homolog, hfzo1, hfzo2, MFN1, mitofusin 1, Transmembrane GTPase MFN1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66776-1-PBS targets MFN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MFN1 fusion protein Ag4890 相同性解析による交差性が予測される生物種
Full Name mitofusin 1
Calculated molecular weight 741 aa, 84 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC040557
Gene Symbol MFN1
Gene ID (NCBI) 55669
RRIDAB_2882122
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWA4
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria. Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).