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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MFN2 Polyclonal antibody

MFN2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28341-1-AP

Synonyms

CMT2A, CMT2A2, CPRP1, HSG, KIAA0214, MARF, MFN2, mitofusin 2, Transmembrane GTPase MFN2

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MFN2 Polyclonal antibody (28341-1-AP)

    WB analysis of HepG2 using 28341-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 28341-1-AP (MFN2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MFN2 Polyclonal antibody (28341-1-AP)

    WB analysis using 28341-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28341-1-AP (MFN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MFN2 Polyclonal antibody (28341-1-AP)

    IHC staining of mouse heart using 28341-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28341-1-AP (MFN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using MFN2 Polyclonal antibody (28341-1-AP)

    IHC staining of mouse heart using 28341-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28341-1-AP (MFN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28341-1-AP targets MFN2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFN2 fusion protein Ag28128 相同性解析による交差性が予測される生物種
Full Name mitofusin 2
Calculated molecular weight 757 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC017061
Gene symbol MFN2
Gene ID (NCBI) 9927
RRIDAB_2881116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFN2, also named as CPRP1 and KIAA0214, belongs to the mitofusin family. It is an Essential transmembrane GTPase, which mediates mitochondrial fusion. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression of MFN2 induces the formation of mitochondrial networks. It plays an important role in the regulation of vascular smooth muscle cell proliferation. Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 2A2 (CMT2A2). Defects in MFN2 are the cause of Charcot-Marie-Tooth disease type 6 (CMT6). Ubiquitinated forms of Mfn2 (mono- and polyubiquitinated) are present during mitophagy.

Protocols

Product Specific Protocols
WB protocol for MFN2 antibody 28341-1-APDownload protocol
IHC protocol for MFN2 antibody 28341-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols