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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MFN2 Recombinant antibody

MFN2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A5

Cat no : 82673-2-RR

Synonyms

MARF, KIAA0214, HSG, EC:3.6.5.-, CPRP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MFN2 Recombinant antibody (82673-2-RR)

    WB analysis using 82673-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82673-2-RR (MFN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MFN2 Recombinant antibody (82673-2-RR)

    IHC staining of human colon cancer using 82673-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MFN2 Recombinant antibody (82673-2-RR)

    IHC staining of human colon cancer using 82673-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82673-2-RR (MFN2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MFN2 Recombinant antibody (82673-2-RR)

    IF Staining of HepG2 using 82673-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MFN2 antibody (82673-2-RR, Clone: 2A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82673-2-RR

    Affinity and Kinetic Characterization of 82673-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82673-2-RR against Human MFN2 were performed. The affinity constant is 19.7 nM.

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Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82673-2-RR targets MFN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MFN2 fusion protein Ag2845 相同性解析による交差性が予測される生物種
Full Name mitofusin 2
Calculated molecular weight 757 aa, 86 kDa
Observed molecular weight 86 kDa
GenBank accession numberBC017061
Gene symbol MFN2
Gene ID (NCBI) 9927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mitofusin-2 (MFN2), a mitochondrial membrane protein, is a dynamin-like GTPase embedded in the outer mitochondrial membrane (OMM) which plays a central role in regulating mitochondrial fusion and cell metabolism (PMID: 35399520; 34026850). There are two human mitofusin isoforms MFN1 and MFN2, MFN1 and MFN2 mediate outer membrane fusion, OPA1 is involved in inner membrane fusion, and DRP1 is responsible for mitochondrial fission (PMID: 17084350; 35399520). It has been shown that MFN2 participates in multiple cellular biological processes under physical and pathological conditions. Apart from mitochondria fusion, MFN2 is also involved in mitochondrial-ER contacts (MERCs), mitophagy, cellular apoptosis, and proliferation (PMID: 34026850).

Protocols

Product Specific Protocols
WB protocol for MFN2 antibody 82673-2-RRDownload protocol
IHC protocol for MFN2 antibody 82673-2-RRDownload protocol
IF protocol for MFN2 antibody 82673-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols