MFN2 Recombinant antibody
MFN2 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2A5
Cat no : 82673-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82673-2-RR targets MFN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
Tested Reactivity | human, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MFN2 fusion protein Ag2845 相同性解析による交差性が予測される生物種 |
Full Name | mitofusin 2 |
Calculated molecular weight | 757 aa, 86 kDa |
Observed molecular weight | 86 kDa |
GenBank accession number | BC017061 |
Gene symbol | MFN2 |
Gene ID (NCBI) | 9927 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mitofusin-2 (MFN2), a mitochondrial membrane protein, is a dynamin-like GTPase embedded in the outer mitochondrial membrane (OMM) which plays a central role in regulating mitochondrial fusion and cell metabolism (PMID: 35399520; 34026850). There are two human mitofusin isoforms MFN1 and MFN2, MFN1 and MFN2 mediate outer membrane fusion, OPA1 is involved in inner membrane fusion, and DRP1 is responsible for mitochondrial fission (PMID: 17084350; 35399520). It has been shown that MFN2 participates in multiple cellular biological processes under physical and pathological conditions. Apart from mitochondria fusion, MFN2 is also involved in mitochondrial-ER contacts (MERCs), mitophagy, cellular apoptosis, and proliferation (PMID: 34026850).
Protocols
Product Specific Protocols | |
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WB protocol for MFN2 antibody 82673-2-RR | Download protocol |
IHC protocol for MFN2 antibody 82673-2-RR | Download protocol |
IF protocol for MFN2 antibody 82673-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |