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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

MFSD2 Polyclonal antibody

MFSD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25498-1-AP

Synonyms

FP1147, HMFN0656, MFSD2, MFSD2A, PP10484, PP9177, RP3 342P20.1, UNQ300/PRO341

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MFSD2 Polyclonal antibody (25498-1-AP)

    WB analysis of HepG2 using 25498-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25498-1-AP (MFSD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using MFSD2 Polyclonal antibody (25498-1-AP)

    IHC staining of human placenta using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using MFSD2 Polyclonal antibody (25498-1-AP)

    IHC staining of human placenta using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MFSD2 Polyclonal antibody (25498-1-AP)

    IHC staining of mouse brain using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MFSD2 Polyclonal antibody (25498-1-AP)

    IHC staining of mouse brain using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25498-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using MFSD2 Polyclonal antibody (25498-1-AP)

    IHC staining of human testis using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25498-1-AP (MFSD2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using MFSD2 Polyclonal antibody (25498-1-AP)

    IHC staining of human testis using 25498-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25498-1-AP (MFSD2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman placenta tissue, human testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25498-1-AP is MFSD2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFSD2 fusion protein Ag22232 相同性解析による交差性が予測される生物種
Full Name major facilitator superfamily domain containing 2
Calculated molecular weight 543 aa, 60 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC092414
Gene symbol MFSD2
Gene ID (NCBI) 84879
RRIDAB_2880103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFSD2, also named as MFSD2A, belongs to a large family of presumptive carbohydrate transporters with 10-12 membrane-spanning domains, is located at chromosomal position 1p34.2, and is conserved in evolution. It plays a role in thermogenesis via beta-adrenergic signaling pathway. It may be the main plasma membrane tunicamycin transporter. MFSD2 has three isoforms with MW 50 kDa and 59-60 kDa.

Protocols

Product Specific Protocols
WB protocol for MFSD2 antibody 25498-1-APDownload protocol
IHC protocol for MFSD2 antibody 25498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IF

Neuro Oncol

Wnt signaling regulates MFSD2A-dependent drug delivery through endothelial transcytosis in glioma

Authors - Yuan Xie
View Article