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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MFSD2 Polyclonal antibody, PBS Only

MFSD2 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30555-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MFSD2 Polyclonal antibody (30555-1-AP)

    WB analysis using 30555-1-AP (same clone as 30555-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30555-1-AP (MFSD2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30555-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MFSD2 Polyclonal antibody (30555-1-AP)

    IHC staining of mouse brain using 30555-1-AP (same clone as 30555-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30555-1-AP (MFSD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30555-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MFSD2 Polyclonal antibody (30555-1-AP)

    IHC staining of mouse brain using 30555-1-AP (same clone as 30555-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30555-1-AP (MFSD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30555-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MFSD2 Polyclonal antibody (30555-1-AP)

    IF Staining of mouse brain using 30555-1-AP (same clone as 30555-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (30555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30555-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MFSD2 Polyclonal antibody (30555-1-AP)

    IF Staining of mouse brain using 30555-1-AP (same clone as 30555-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (30555-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30555-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30555-1-PBS targets MFSD2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MFSD2 fusion protein Ag33064 相同性解析による交差性が予測される生物種
Full Name major facilitator superfamily domain containing 2
Calculated molecular weight 543 aa, 60 kDa
Observed molecular weight55-60 kDa
GenBank accession numberBC092414
Gene symbol MFSD2
Gene ID (NCBI) 84879
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MFSD2 is a transmembrane protein and sodium-dependent lysophosphatidylcholine transporter. MFSD2 is involved in the establishment of the blood-brain barrier and is required for brain growth and function.