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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MFSD2 Monoclonal antibody

MFSD2 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2D2B4

Cat no : 67965-1-Ig

Synonyms

MFSD2a, Major facilitator superfamily domain-containing protein 2A, HsMFSD2A, HMFN0656, FP1147

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using MFSD2 Monoclonal antibody (67965-1-Ig)

    WB analysis of human testis using 67965-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MFSD2 Monoclonal antibody (67965-1-Ig)

    WB analysis of human placenta using 67965-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using MFSD2 Monoclonal antibody (67965-1-Ig)

    WB analysis of human testis using 67965-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using MFSD2 Monoclonal antibody (67965-1-Ig)

    WB analysis of human placenta using 67965-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67965-1-Ig (MFSD2 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using MFSD2 Monoclonal antibody (67965-1-Ig)

    IF Staining of mouse brain using 67965-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using MFSD2 antibody (67965-1-Ig, Clone: 2D2B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inhuman placenta tissue, human testis tissue
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67965-1-Ig targets MFSD2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MFSD2 fusion protein Ag31028 相同性解析による交差性が予測される生物種
Full Name major facilitator superfamily domain containing 2
Calculated molecular weight 543 aa, 60 kDa
Observed molecular weight60 kDa
GenBank accession numberBC092414
Gene symbol MFSD2
Gene ID (NCBI) 84879
RRIDAB_2918716
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MFSD2, also named as MFSD2A, belongs to a large family of presumptive carbohydrate transporters with 10-12 membrane-spanning domains, is located at chromosomal position 1p34.2, and is conserved in evolution. It plays a role in thermogenesis via beta-adrenergic signaling pathway. It may be the main plasma membrane tunicamycin transporter. MFSD2 has three isoforms with MW 50 kDa and 59-60 kDa.

Protocols

Product Specific Protocols
WB protocol for MFSD2 antibody 67965-1-IgDownload protocol
IF protocol for MFSD2 antibody 67965-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Food Chem

Dietary sn-2 palmitate influences cognitive behavior by increasing the transport of liver-produced lysophosphatidylcholine VLCPUFAs to the brain

Authors - Teng Wei
View Article