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MGAT1 Polyclonal antibody

MGAT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15103-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

GlcNAc-T I, GLCNAC TI, GlcNAc T I, GGNT1, EC:2.4.1.101

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inY79 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue, mouse heart tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15103-1-AP targets MGAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGAT1 fusion protein Ag7446 相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Calculated molecular weight 39 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC003575
Gene Symbol MGAT1
Gene ID (NCBI) 4245
RRIDAB_2297618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26572
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MGAT1 antibody 15103-1-APDownload protocol
IHC protocol for MGAT1 antibody 15103-1-APDownload protocol
IF protocol for MGAT1 antibody 15103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Arthritis Rheum

Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage.

Authors - Urita Atsushi A
mouseWB

FASEB J

Reduced myocyte complex N-glycosylation causes dilated cardiomyopathy.

Authors - Andrew R Ednie
human, ratWB

Schizophr Res

Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia.

Authors - Jordyn M Kippe
mouseWB

Liver Transpl

Monoacylglycerol Acyltransferase 1 Knockdown Exacerbates Hepatic Ischemia/Reperfusion Injury in Mice With Hepatic Steatosis.

Authors - Kim H H Liss
ratWB,IF

Sci Rep

Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats.

Authors - Kaimei Lu
humanWB

Biochemistry

N-linked glycosylation dependent and independent mechanisms regulating CTRP12 cleavage, secretion, and stability.

Authors - Ashley N Stewart
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