Validation Data Gallery
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Tested Applications
| Positive WB detected in | Y79 cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human heart tissue, mouse heart tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
15103-1-AP targets MGAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MGAT1 fusion protein Ag7446 相同性解析による交差性が予測される生物種 |
| Full Name | mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 51-55 kDa |
| GenBank accession number | BC003575 |
| Gene Symbol | MGAT1 |
| Gene ID (NCBI) | 4245 |
| RRID | AB_2297618 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26572 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MGAT1 antibody 15103-1-AP | Download protocol |
| IHC protocol for MGAT1 antibody 15103-1-AP | Download protocol |
| IF protocol for MGAT1 antibody 15103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Arthritis Rheum Alterations of high-mannose type N-glycosylation in human and mouse osteoarthritis cartilage. | ||
Schizophr Res Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia. | ||
Liver Transpl Monoacylglycerol Acyltransferase 1 Knockdown Exacerbates Hepatic Ischemia/Reperfusion Injury in Mice With Hepatic Steatosis. | ||
Sci Rep Bupivacaine reduces GlyT1 expression by potentiating the p-AMPKα/BDNF signalling pathway in spinal astrocytes of rats. | ||
Biochemistry N-linked glycosylation dependent and independent mechanisms regulating CTRP12 cleavage, secretion, and stability. |