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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MGAT2 Polyclonal antibody

MGAT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15772-1-AP

Synonyms

CDGS2, GlcNAc T II, GLCNACTII, GNT II, GNT2, MGAT2

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using MGAT2 Polyclonal antibody (15772-1-AP)

    WB analysis of human liver using 15772-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15772-1-AP (MGAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using MGAT2 Polyclonal antibody (15772-1-AP)

    WB analysis of mouse heart using 15772-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15772-1-AP (MGAT2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using MGAT2 Polyclonal antibody (15772-1-AP)

    IHC staining of human liver using 15772-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15772-1-AP (MGAT2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using MGAT2 Polyclonal antibody (15772-1-AP)

    IHC staining of human liver using 15772-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15772-1-AP (MGAT2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inhuman liver tissue, mouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15772-1-AP targets MGAT2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGAT2 fusion protein Ag8423 相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Calculated molecular weight 447 aa, 52 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC006390
Gene symbol MGAT2
Gene ID (NCBI) 4247
RRIDAB_2142907
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MGAT2 antibody 15772-1-APDownload protocol
IHC protocol for MGAT2 antibody 15772-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols