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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using MGAT4A Polyclonal antibody (25109-1-AP)

    WB analysis of HeLa using 25109-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25109-1-AP (MGAT4A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MGAT4A Polyclonal antibody (25109-1-AP)

    IHC staining of mouse small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25109-1-AP (MGAT4A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using MGAT4A Polyclonal antibody (25109-1-AP)

    IHC staining of mouse small intestine using 25109-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 25109-1-AP (MGAT4A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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MGAT4A Polyclonal antibody

MGAT4A Polyclonal Antibody for WB, ELISA
Cat No. 25109-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

GNT IVA, GNT IV, GlcNAc-T IVa, GlcNAc T IVa, EC:2.4.1.145

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25109-1-AP targets MGAT4A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGAT4A fusion protein Ag18956 相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A
Calculated molecular weight 535 aa, 62 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC127107
Gene Symbol MGAT4A
Gene ID (NCBI) 11320
RRIDAB_2879901
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UM21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MGAT4A antibody 25109-1-APDownload protocol
IHC protocol for MGAT4A antibody 25109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Biochim Biophys Acta Mol Cell Res

N-glycosylation by N-acetylglucosaminyltransferase IVa enhances the interaction of integrin β1 with vimentin and promotes hepatocellular carcinoma cell motility

Authors - Depeng Yang
View Article