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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MGAT4B Polyclonal antibody

MGAT4B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30718-1-AP

Synonyms

GnT-IVb, GNT IVB, GNT IV, GlcNAc-T IVb, EC:2.4.1.145

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGAT4B Polyclonal antibody (30718-1-AP)

    WB analysis using 30718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MGAT4B Polyclonal antibody (30718-1-AP)

    WB analysis using 30718-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30718-1-AP (MGAT4B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MGAT4B Polyclonal antibody (30718-1-AP)

    IHC staining of mouse liver using 30718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using MGAT4B Polyclonal antibody (30718-1-AP)

    IHC staining of mouse liver using 30718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MGAT4B Polyclonal antibody (30718-1-AP)

    IHC staining of mouse kidney using 30718-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30718-1-AP (MGAT4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MGAT4B Polyclonal antibody (30718-1-AP)

    IF Staining of U2OS using 30718-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MGAT4B antibody (30718-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected in37°C incubated mouse liver tissue, human liver tissue
Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30718-1-AP targets MGAT4B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGAT4B fusion protein Ag33769 相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme B
Calculated molecular weight 548 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC009464
Gene symbol MGAT4B
Gene ID (NCBI) 11282
RRIDAB_3086399
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MGAT4B belongs to the N-Acetylglucosaminyltransferase-IV family and catalyzes the transfer of GlcNAc from UDP-GlcNAc to the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans through a beta1-4 linkage and participates in the production of tri- and tetra-antennary N-linked sugar chains (PMID: 17006639, 10372966). MGAT4B has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor to N-glycan biosynthesis (PMID:17006639).

Protocols

Product Specific Protocols
WB protocol for MGAT4B antibody 30718-1-APDownload protocol
IHC protocol for MGAT4B antibody 30718-1-APDownload protocol
IF protocol for MGAT4B antibody 30718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols