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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MGAT5B Polyclonal antibody

MGAT5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16993-1-AP

Synonyms

GlcNAc T Vb, GnT IX, GnT VB, hGnTVb, KIAA2008, MGAT5B

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of HepG2 using 16993-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of SH-SY5Y using 16993-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of MCF-7 using 16993-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of Jurkat using 16993-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    WB analysis of C6 using 16993-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 16993-1-AP (MGAT5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using MGAT5B Polyclonal antibody (16993-1-AP)

    IP experiment of Jurkat using 16993-1-AP

    IP result of anti-MGAT5B (IP:16993-1-AP, 4ug; Detection:16993-1-AP 1:700) with Jurkat cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using MGAT5B Polyclonal antibody (16993-1-AP)

    IHC staining of mouse brain using 16993-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16993-1-AP (MGAT5B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MGAT5B Polyclonal antibody (16993-1-AP)

    IF Staining of HepG2 using 16993-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 16993-1-AP (MGAT5B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, SH-SY5Y cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 16993-1-AP is MGAT5B Fusion Protein expressed in E. coli.

Tested Reactivity human, rat, mouse
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGAT5B fusion protein Ag10678 相同性解析による交差性が予測される生物種
Full Name mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B
Calculated molecular weight 394 aa, 44 kDa, 89 kDa
Observed molecular weight 89-100 kDa
GenBank accession numberBC062354
Gene symbol MGAT5B
Gene ID (NCBI) 146664
RRIDAB_10858919
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MGAT5B antibody 16993-1-APDownload protocol
IHC protocol for MGAT5B antibody 16993-1-APDownload protocol
IF protocol for MGAT5B antibody 16993-1-APDownload protocol
IP protocol for MGAT5B antibody 16993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, ratWB

Schizophr Res

Abnormal N-acetylglucosaminyltransferase expression in prefrontal cortex in schizophrenia.

Authors - Jordyn M Kippe
View Article
humanIHC

Medicine (Baltimore)

Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma

Authors - Jia Liu
View Article
humanWB,IF

J Biol Chem

Brain-specific glycosylation enzyme GnT-IX maintains levels of protein tyrosine phosphatase receptor PTPRZ, thereby mediating glioma growth

Authors - Kenichiro Nagai
View Article