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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MGC33407/ACTL9 Polyclonal antibody

MGC33407/ACTL9 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29885-1-AP

Synonyms

Actin like protein 9, ACTL7C, ACTL9, HSD21, hypothetical protein MGC33407

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    WB analysis using 29885-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of human liver using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of human liver using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of mouse testis using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IHC staining of mouse testis using 29885-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29885-1-AP (MGC33407/ACTL9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IF Staining of mouse testis using 29885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using MGC33407/ACTL9 Polyclonal antibody (29885-1-AP)

    IF Staining of mouse testis using 29885-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using MGC33407/ACTL9 antibody (29885-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle
Positive IHC detected inhuman liver tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29885-1-AP targets MGC33407/ACTL9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MGC33407/ACTL9 fusion protein Ag31805 相同性解析による交差性が予測される生物種
Full Name hypothetical protein MGC33407
Observed molecular weight45 kDa
GenBank accession numberBC024028
Gene symbol ACTL9
Gene ID (NCBI) 284382
RRIDAB_2923617
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTL9 (Actin Like 9), also named MGC33407, encodes a testis-specific actin-like protein that plays a role in fusion of proacrosomal vesicles and perinuclear theca formation (PMID: 33626338). ACTL9 protein contains 416 amino acids, which mainly localizes in the equatorial segment of human sperm head (PubMed: 33626338). Diseases associated with ACTL9 include spermatogenic failure and non-syndromic male infertility.

Protocols

Product Specific Protocols
WB protocol for MGC33407/ACTL9 antibody 29885-1-APDownload protocol
IHC protocol for MGC33407/ACTL9 antibody 29885-1-APDownload protocol
IF protocol for MGC33407/ACTL9 antibody 29885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols