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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGEA5 Recombinant antibody (84722-4-RR)

    WB analysis using 84722-4-RR (same clone as 84722-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84722-4-RR (MGEA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using MGEA5 Recombinant antibody (84722-4-RR)

    IHC staining of human rectal cancer using 84722-4-RR (same clone as 84722-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 84722-4-RR (MGEA5 antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MGEA5 Recombinant antibody (84722-4-RR)

    IF Staining of HepG2 using 84722-4-RR (same clone as 84722-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (84722-4-RR, Clone: 242263A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84722-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84722-4-RR

    Affinity and Kinetic Characterization of 84722-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84722-4-RR against Human MGEA5 were performed. The affinity constant is below 1 pM.

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MGEA5 Recombinant antibody, PBS Only

MGEA5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84722-4-PBS
Clone No.242263A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

OGA, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase, Beta-N-acetylglucosaminidase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84722-4-PBS targets MGEA5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MGEA5 fusion protein Ag6405 相同性解析による交差性が予測される生物種
Full Name meningioma expressed antigen 5 (hyaluronidase)
Calculated molecular weight 103 kDa
Observed molecular weight 130-140 kDa
GenBank accession numberBC039583
Gene Symbol MGEA5
Gene ID (NCBI) 10724
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO60502
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).