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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MGLL Monoclonal antibody (67846-1-Ig)

    WB analysis using 67846-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67846-1-Ig (MGLL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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MGLL Monoclonal antibody

MGLL Monoclonal Antibody for WB, ELISA
Cat No. 67846-1-Ig
Clone No.2A3E11

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig

Applications

WB, ELISA

Synonyms

HU K5, HUK5, Lysophospholipase homolog, Lysophospholipase like, MGL, MGLL, monoglyceride lipase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, A549 cells, HeLa cells, human platelets tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67846-1-Ig targets MGLL in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MGLL fusion protein Ag7052 相同性解析による交差性が予測される生物種
Full Name monoglyceride lipase
Calculated molecular weight 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC000551
Gene Symbol MGLL
Gene ID (NCBI) 11343
RRIDAB_2918608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MGLL (monoglyceride lipase) is also named as MAGL, HU-K5 and belongs to the monoacylglycerol lipase family. It converts monoacylglycerides to free fatty acids and glycerol and functions together with hormone-sensitive lipase (HSL) in the mobilization of fatty acids from the triglyceride stores of adipocytes. MGL protein is ubiquitously expressed in kidney, spleen, heart, liver, stomach, brain, lung, adrenal gland, adipose tissue and in brain,there are two bands of 33 kDa and 35 kDa can be detected. The single protein species expressed in muscle is larger (around 40 kDa) (PMID:11470505). This protein can exsit as a homodimer (PMID:19957260).

Protocols

Product Specific Protocols
WB protocol for MGLL antibody 67846-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols