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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MGP Monoclonal antibody, PBS Only

MGP Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1C3

Cat no : 60055-1-PBS

Synonyms

GIG36, matrix Gla protein, MGLAP, MGP, NTI

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using MGP Monoclonal antibody (60055-1-Ig)

    WB analysis of human placenta using 60055-1-Ig (same clone as 60055-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using MGP Monoclonal antibody (60055-1-Ig)

    WB analysis of human kidney using 60055-1-Ig (same clone as 60055-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using MGP Monoclonal antibody (60055-1-Ig)

    WB analysis of human brain using 60055-1-Ig (same clone as 60055-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human saliva using MGP Monoclonal antibody (60055-1-Ig)

    WB analysis of human saliva using 60055-1-Ig (same clone as 60055-1-PBS)

    human saliva were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver cancer tissue using MGP Monoclonal antibody (60055-1-Ig)

    WB analysis of human liver cancer using 60055-1-Ig (same clone as 60055-1-PBS)

    human liver cancer tissue were subjected to SDS PAGE followed by western blot with 60055-1-Ig (MGP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human heart using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human heart using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human kidney using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human kidney using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60055-1-Ig (MGP Antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human brain using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human brain using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human lung using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human lung using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using MGP Monoclonal antibody (60055-1-Ig)

    IHC staining of human gliomas using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 60055-1-Ig(MGP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using MGP Monoclonal antibody (60055-1-Ig)

    IF Staining of mouse kidney using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (60055-1-Ig, Clone: 1A1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDA-MB-231 cells using MGP Monoclonal antibody (60055-1-Ig)

    IF Staining of MDA-MB-231 using 60055-1-Ig (same clone as 60055-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using MGP antibody (60055-1-Ig, Clone: 1A1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60055-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60055-1-PBS targets MGP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen MGP fusion protein Ag1091 相同性解析による交差性が予測される生物種
Full Name matrix Gla protein
Calculated molecular weight 103 aa, 13 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC005272
Gene symbol MGP
Gene ID (NCBI) 4256
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.