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Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 647 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

M5/114.15.2

Cat no : CL647-65122

Synonyms

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II (I-A/I-E)

Validation Data Gallery

Applications:
  • IF Staining of mouse splenocytes using CL647-65122

    IF Staining of mouse splenocytes using CL647-65122

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse MHC Class II (I-A/I-E) (CL647-65122, Clone: M5/114.15.2) at dilution of 1:400.

  • FC experiment of mouse splenocytes using CL647-65122

    FC experiment of mouse splenocytes using CL647-65122

    1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite®488 Anti-Mouse CD3 (17A2) (CL488-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated Rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 647 Anti-Mouse MHC Class II (I-A/I-E) (CL647-65122, Clone: M5/114.15.2) (right). Cells were not fixed.

Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65122-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

CL647-65122 targets MHC Class II (I-A/I-E) in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Activated C57BL/6 mouse spleen cells
Full Name histocompatibility 2, class II antigen A, alpha
GenBank accession numberBC031711
Gene symbol H2-Aa
Gene ID (NCBI) 14960
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 665 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 MHC Class II (I-A/I-E) antibody CL647-65122Download protocol
FC protocol for CL Plus 647 MHC Class II (I-A/I-E) antibody CL647-65122Download protocol
Standard Protocols
Click here to view our Standard Protocols