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Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC
Cat No. 65122-1-Ig
Clone No.M5/114.15.2

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

IF, FC

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 2 publications below

Product Information

65122-1-Ig targets MHC Class II (I-A/I-E) in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Cited Reactivity mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Activated C57BL/6 mouse spleen cells 相同性解析による交差性が予測される生物種
Full Name histocompatibility 2, class II antigen A, alpha
GenBank accession numberBC031711
Gene Symbol MHC Class II
Gene ID (NCBI) 14960
RRIDAB_2918416
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP14434
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

Protocols

Product Specific Protocols
FC protocol for MHC Class II (I-A/I-E) antibody 65122-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Int Immunopharmacol

Targeted inhibition of the immunoproteasome blocks endothelial MHC class II antigen presentation to CD4+ T cells in chronic liver injury

Authors - Yuwei Zhang
mouseIF

J Ethnopharmacol

Jin-Gui-Shen-Qi Wan alleviates fibrosis in mouse diabetic nephropathy via MHC class II

Authors - Dan Liang
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