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- KD/KO Validated
MIA3 Polyclonal antibody
MIA3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 17481-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 17481-1-AP is MIA3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIA3 fusion protein Ag11514 相同性解析による交差性が予測される生物種 |
| Full Name | melanoma inhibitory activity family, member 3 |
| Calculated molecular weight | 214 kDa |
| Observed molecular weight | 300 kDa |
| GenBank accession number | BC047116 |
| Gene symbol | MIA3 |
| Gene ID (NCBI) | 375056 |
| RRID | AB_2878411 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Melanoma inhibitory activity member 3 (MIA3/TANGO1) is an evolutionarily conserved endoplasmic reticulum resident transmembrane protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIA3 antibody 17481-1-AP | Download protocol |
| IHC protocol for MIA3 antibody 17481-1-AP | Download protocol |
| IP protocol for MIA3 antibody 17481-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Endocrinol (Lausanne) MIA SH3 Domain ER Export Factor 3 Deficiency Prevents Neointimal Formation by Restoring BAT-Like PVAT and Decreasing VSMC Proliferation and Migration.
| ||
Arthritis Rheumatol Caspase-1 Enhances TANGO1 Expression to Promote Procollagen Export from the Endoplasmic Reticulum in Systemic Sclerosis Contributing to Fibrosis
| ||
Elife Dynamic regulation of inter-organelle communication by ubiquitylation controls skeletal muscle development and disease onset | ||
Mol Cell Biochem MIA3 promotes the degradation of GSH (glutathione) by binding to CHAC1, thereby promoting the progression of hepatocellular carcinoma
| ||
Dev Cell Endoplasmic reticulum exit sites are segregated for secretion based on cargo size |
