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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIB1 Recombinant antibody (83718-5-RR)

    WB analysis using 83718-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83718-5-RR (MIB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using MIB1 Recombinant antibody (83718-5-RR)

    FC experiment of HeLa using 83718-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using MIB1 Recombinant antibody (83718-5-RR)

    FC experiment of SH-SY5Y using 83718-5-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug MIB1 Recombinant antibody (83718-5-RR, Clone:240676E4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83718-5-RR

    Affinity and Kinetic Characterization of 83718-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83718-5-RR against Human MIB1 were performed. The affinity constant is 5.15 nM.

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MIB1 Recombinant antibody

MIB1 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83718-5-RR
Clone No.240676E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

DIP-1, DIP1, DIP 1, DAPK-interacting protein 1, DAPK interacting protein 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, DU 145 cells, HEK-293 cells, K-562 cells, C6 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83718-5-RR targets MIB1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MIB1 fusion protein Ag2507 相同性解析による交差性が予測される生物種
Full Name mindbomb homolog 1 (Drosophila)
Calculated molecular weight 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC022403
Gene Symbol MIB1
Gene ID (NCBI) 57534
RRIDAB_3671320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ86YT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MIB1(Mind bomb homolog 1) is an E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. MIB1, identified as an interactor of death associated protein kinase (DAPK), probably regulate apoptosis through ubiquitination and degradation of DAPK, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. MIB1 promotes proteasomal degradation of NRF2 and sensitizes lung cancer cells to ferroptosis(PMID: 34670864). In addition, MIB1 upregulates IQGAP1 and promotes pancreatic cancer progression by inducing ST7 degradation(PMID: 33793053).

Protocols

Product Specific Protocols
WB protocol for MIB1 antibody 83718-5-RRDownload protocol
FC protocol for MIB1 antibody 83718-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols