Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
13696-1-AP targets MIB2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIB2 fusion protein Ag4592 相同性解析による交差性が予測される生物種 |
| Full Name | mindbomb homolog 2 (Drosophila) |
| Calculated molecular weight | 110 kDa |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC037542 |
| Gene Symbol | MIB2 |
| Gene ID (NCBI) | 142678 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AX9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MIB2, also named as E3 ubiquitin-protein ligase MIB2, is a 1013 amino aicd protein, which Contains 9 ANK repeats and 2 MIB/HERC2 domains. MIB2 as E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins.MIB2 positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. MIB2 is expressed in skeletal muscle, and to a lesser extent in heart, brain and kidney.
Publications
| Species | Application | Title |
|---|---|---|
PNAS Nexus Mind bomb 2 limits inflammatory dermatitis in Sharpin mutant mice independently of cell death |