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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MICA/MICB Recombinant antibody (83725-5-RR)

    WB analysis using 83725-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83725-5-RR (MICA/MICB antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83725-5-RR

    Affinity and Kinetic Characterization of 83725-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83725-5-RR against Human MICA were performed. The affinity constant is 1.55 nM.

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MICA/MICB Recombinant antibody

MICA/MICB Recombinant Antibody for WB, ELISA
Cat No. 83725-5-RR
Clone No.240735D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

MICB, MICA, PERB11.1, MIC A, 240735D9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83725-5-RR targets MICA/MICB in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name MHC class I polypeptide-related sequence A
Calculated molecular weight43 kDa
Observed molecular weight50-60 kDa
GenBank accession numberAAH16929.1
Gene Symbol MICA
Gene ID (NCBI) 100507436
RRIDAB_3671325
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.

Protocols

Product Specific Protocols
WB protocol for MICA/MICB antibody 83725-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols