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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human MICA/MICB Rabbit Recombinant Antibody

MICA/MICB Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240735D5

Cat no : 98110-1-RR

Synonyms

MICB, MICA, PERB11.1, MIC A, 240735D5

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using Anti-Human MICA/MICB Rabbit Recombinant Antibody (98110-1-RR)

    FC experiment of HeLa using 98110-1-RR

    1x10^6 HeLa cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human MICA/MICB Rabbit Recombinant Antibody (98110-1-RR, Clone: 240735D5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98110-1-RR

    Affinity and Kinetic Characterization of 98110-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98110-1-RR against Human MICA were performed. The affinity constant is 0.907 nM.

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Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98110-1-RR is MICA/MICB Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name MHC class I polypeptide-related sequence A
Calculated molecular weight43 kDa
GenBank accession numberAAH16929.1
Gene symbol MICA
Gene ID (NCBI) 4276
RRIDAB_3672257
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). This antibody recognizes both MICA and MICB.

Protocols

Product Specific Protocols
FC protocol for MICA/MICB antibody 98110-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols