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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MID2 Monoclonal antibody

MID2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F9E4

Cat no : 68359-1-Ig

Synonyms

FLJ37715, FLJ41813, FXY2, MID2, Midin 2, midline 2, Midline defect 2, RING finger protein 60, RNF60, TRIM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MID2 Monoclonal antibody (68359-1-Ig)

    WB analysis using 68359-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MID2 Monoclonal antibody (68359-1-Ig)

    WB analysis of Jurkat using 68359-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68359-1-Ig (MID2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, Jurkat cells, MCF-7 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68359-1-Ig targets MID2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen MID2 fusion protein Ag30793 相同性解析による交差性が予測される生物種
Full Name midline 2
Calculated molecular weight 715 aa, 81 kDa
Observed molecular weight80~83 kDa
GenBank accession numberBC017707
Gene symbol MID2
Gene ID (NCBI) 11043
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Midline2 (MID2) is an ubiquitin-conjugating E2 enzyme that is involved in tumor development and has a physical interaction with breast cancer 1,early-onset (BRCA1).

Protocols

Product Specific Protocols
WB protocol for MID2 antibody 68359-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogenesis

An E3 ligase TRIM1 promotes colorectal cancer progression via K63-linked ubiquitination and activation of HIF1α

Authors - Liuliu Shi
View Article