- Featured Product
- KD/KO Validated
MIDN Polyclonal antibody
MIDN Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 18939-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
18939-1-AP targets MIDN in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIDN fusion protein Ag13492 相同性解析による交差性が予測される生物種 |
| Full Name | midnolin |
| Calculated molecular weight | 468 aa, 49 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC094778 |
| Gene symbol | MIDN |
| Gene ID (NCBI) | 90007 |
| RRID | AB_2878569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Midbrain nucleolar protein (midnolin, MIDN) is abundantly expressed in embryonic midbrain and localizes in the nucleus and nucleolus. MIDN mRNA was strongly and abundantly expressed during early developmental stages, and that the expression was not limited to midbrain but also expressed in diverse adult mouse tissues, including heart, spleen, lung, liver, skeletal muscle, kidney and testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIDN antibody 18939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Biol CREB promotes beta cell gene expression by targeting Its coactivators to tissue-specific enhancers. | ||
Science The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation
| ||
Cancer Sci Exosomal miR-222-3p contributes to castration-resistant prostate cancer by activating mTOR signaling |
