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KD/KO Validated

MIF Polyclonal antibody

Name: MIF Polyclonal antibody
Brand: Proteintech
SKU: 20415-1-AP
Rating: 4.60 (5 reviews)

MIF Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (5)

Cat no : 20415-1-AP

Synonyms

Macrophage migration inhibitory factor, L-dopachrome tautomerase, L-dopachrome isomerase, Glycosylation-inhibiting factor, EC:5.3.3.12

Validation Data Gallery

Filter:

AllWBIPIF/ICCFC (Intra)

WB analysis using 20415-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 20415-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 20415-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis using 20415-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 20415-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 20415-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 20415-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse spleen using 20415-1-AP
IP result of anti-MIF (IP:20415-1-AP, 4ug; Detection:20415-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.
IF Staining of U-251 using 20415-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MIF antibody (20415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of THP-1 using 20415-1-AP
1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human MIF (20415-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 20415-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, U-937 cells, Y79 cells, HL-60 cells, Jurkat cells, U-87 MG cells, HEK-293T cells, HUVEC cells, J774A.1 cells, Neuro-2a cells, mouse spleen tissue, rat spleen tissue
Positive IP detected in	mouse spleen tissue
Positive IF/ICC detected in	U-251 cells
Positive FC (Intra) detected in	THP-1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 13 publications below
IF	See 7 publications below

Product Information

20415-1-AP targets MIF in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MIF fusion protein Ag14058 相同性解析による交差性が予測される生物種
Full Name	macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Calculated molecular weight	115 aa, 12 kDa
Observed molecular weight	12 kDa
GenBank accession number	BC000447
Gene symbol	MIF
Gene ID (NCBI)	4282
RRID	AB_10694820
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.

Protocols

Product Specific Protocols
WB protocol for MIF antibody 20415-1-AP	Download protocol
IF protocol for MIF antibody 20415-1-AP	Download protocol
IP protocol for MIF antibody 20415-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Stem Cell Res Ther DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis Authors - S Y Wang View Article
human	WB	Cancer Lett Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β. Authors - Rui-Min Liu KD Validated View Article
human	WB	Cell Prolif TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD). Authors - Xiaojun Zhang View Article
mouse	WB	Front Endocrinol (Lausanne) Exercise prevents fatal stress-induced myocardial injury in obese mice Authors - Yaoshan Dun View Article
mouse	IF	Food Chem Toxicol The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation Authors - Siwen Li View Article
human	WB	Mol Carcinog Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9. Authors - Sha-Sha Wang KD Validated View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH Tianyi (Verified Customer) (01-12-2024)	Antigen retrieval in TE buffer, incubated with AF594 for 2 h at RT Applications: Immunofluorescence Primary Antibody Dilution: 1:200 Cell Tissue Type: Ovarian tissue MIF Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:20415-1-AP)
FH sarah (Verified Customer) (10-27-2023)	antibody worked great for WB Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: 293T, HCT116
FH Sarah (Verified Customer) (01-24-2023)	This antibody worked well despite low protein concentration. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: HCT116
FH Emma (Verified Customer) (03-15-2022)	Nice antibody have used overnight @ 1:1000 on cell lysates and conditioned media. Produces a band at the correct size. Applications: Western Blot Primary Antibody Dilution: 1:1000 Cell Tissue Type: PC3
FH Ryan (Verified Customer) (02-28-2019)	Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown). Applications: Immunohistochemistry, Immunofluorescence, Primary Antibody Dilution: 1:100 Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf MIF Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:20415-1-AP)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MIF Polyclonal antibody

MIF Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 20415-1-AP

WB analysis using 20415-1-AP

WB analysis using 20415-1-AP

WB analysis using 20415-1-AP

WB analysis using 20415-1-AP

WB analysis of U-937 using 20415-1-AP

WB analysis of Y79 using 20415-1-AP

IP experiment of mouse spleen using 20415-1-AP

IF Staining of U-251 using 20415-1-AP

FC experiment of THP-1 using 20415-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

DPSCs regulate epithelial-T cell interactions in oral submucous fibrosis

Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD).

Exercise prevents fatal stress-induced myocardial injury in obese mice

The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation

Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.

Reviews

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