MIF Polyclonal antibody
MIF Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 30957-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | J774A.1 cells, NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse brain tissue, mouse kidney tissue |
Positive IF/ICC detected in | Neuro-2a cells, NIH/3T3 cells |
Positive FC (Intra) detected in | NIH/3T3 cells |
Positive FC detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
30957-1-AP targets MIF in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with mouse, rat samples.
Tested Reactivity | mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
Full Name | macrophage migration inhibitory factor |
Calculated molecular weight | 13KD |
Observed molecular weight | 13 kDa |
GenBank accession number | NM_010798.2 |
Gene symbol | Mif |
Gene ID (NCBI) | 17319 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MIF is a pleiotropic cytokine that contributes to the pathogenesis of many autoimmune diseases through its upstream immunoregulatory function and its polymorphic genetic locus. MIF is a highly conserved protein of 12.5 kDa, with evolutionarily ancient homologues in plants, protozoans, nematodes, and invertebrates.
Protocols
Product Specific Protocols | |
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WB protocol for MIF antibody 30957-1-AP | Download protocol |
IF protocol for MIF antibody 30957-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |