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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MIG6; ERRFI1 Recombinant antibody

MIG6; ERRFI1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240447C3

Cat no : 83475-2-RR

Synonyms

ERRFI1, 240447C3, MIG-6, MIG6, MIG 6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIG6; ERRFI1 Recombinant antibody (83475-2-RR)

    WB analysis using 83475-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83475-2-RR (MIG6; ERRFI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HepG2 cells using MIG6; ERRFI1 Recombinant antibody (83475-2-RR)

    FC experiment of HepG2 using 83475-2-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug MIG6; ERRFI1 Recombinant antibody (83475-2-RR, Clone:240447C3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83475-2-RR

    Affinity and Kinetic Characterization of 83475-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83475-2-RR against Human MIG6; ERRFI1 were performed. The affinity constant is 2.04 nM.

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Tested Applications

Positive WB detected inHCT 116 cells, HepG2
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83475-2-RR is MIG6; ERRFI1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MIG6; ERRFI1 fusion protein Ag33341 相同性解析による交差性が予測される生物種
Full Name ERBB receptor feedback inhibitor 1
Calculated molecular weight51kd
Observed molecular weight 51 kDa
GenBank accession numberNM_018948
Gene symbol MIG6
Gene ID (NCBI) 54206
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for MIG6; ERRFI1 antibody 83475-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols