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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MIOS Recombinant antibody, PBS Only

MIOS Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240832D12

Cat no : 83856-4-PBS

Synonyms

Missing oocyte meiosis regulator homolog, GATOR2 complex protein MIOS, 240832D12

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MIOS Recombinant antibody (83856-4-RR)

    IF Staining of U2OS using 83856-4-RR (same clone as 83856-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MIOS antibody (83856-4-RR, Clone: 240832D12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83856-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83856-4-RR

    Affinity and Kinetic Characterization of 83856-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83856-4-RR against Human MIOS were performed. The affinity constant is 0.439 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83856-4-PBS is MIOS Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MIOS fusion protein Ag15148 相同性解析による交差性が予測される生物種
Full Name missing oocyte, meiosis regulator, homolog (Drosophila)
Calculated molecular weight 875 aa, 99 kDa
GenBank accession numberBC005883
Gene symbol MIOS
Gene ID (NCBI) 54468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The mechanistic target of rapamycin complex 1 (mTORC1) controls growth by regulating anabolic and catabolic processes in response to environmental cues, including nutrients. Amino acids signal to mTORC1 through the Rag GTPases, which are regulated by several protein complexes, including GATOR1 and GATOR2. GATOR2, which has five components (WDR24, MIOS, WDR59, SEH1L, SEC13), is required for amino acids to activate mTORC1. PMID: 3583151