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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIP-1 alpha/CCL3 Polyclonal antibody (27441-1-AP)

    WB analysis using 27441-1-AP

    PMA, LPS, and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 27441-1-AP (MIP 1 alpha/CCL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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MIP-1 alpha/CCL3 Polyclonal antibody

MIP-1 alpha/CCL3 Polyclonal Antibody for WB, ELISA
Cat No. 27441-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

C C motif chemokine 3, CCL 3, CCL3, CCL-3, chemokine (C C motif) ligand 3, G0S19 1, MIP 1 alpha, MIP 1 alpha/CCL3, MIP 1A, MIP-1 alpha, MIP1A, MIP-1α

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27441-1-AP targets MIP-1 alpha/CCL3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIP-1 alpha/CCL3 fusion protein Ag26764 相同性解析による交差性が予測される生物種
Full Name chemokine (C-C motif) ligand 3
Calculated molecular weight 92 aa, 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC071834
Gene Symbol CCL3/MIP-1 alpha
Gene ID (NCBI) 6348
RRIDAB_3085961
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10147
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1α, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.

Protocols

Product Specific Protocols
WB protocol for MIP-1 alpha/CCL3 antibody 27441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols