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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MIPEP Polyclonal antibody (11002-1-AP)

    WB analysis of HepG2 using 11002-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11002-1-AP (MIPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MIPEP Polyclonal antibody (11002-1-AP)

    WB analysis of MCF-7 using 11002-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11002-1-AP (MIPEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MIPEP Polyclonal antibody (11002-1-AP)

    IHC staining of human prostate cancer using 11002-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11002-1-AP (MIPEP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MIPEP Polyclonal antibody (11002-1-AP)

    IHC staining of human prostate cancer using 11002-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11002-1-AP (MIPEP Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MIPEP Polyclonal antibody (11002-1-AP)

    IHC staining of human prostate cancer using 11002-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 11002-1-AP (MIPEP Antibody) at dilution of 1:50.

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MIPEP Polyclonal antibody

MIPEP Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11002-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HMIP, MIP, MIPEP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11002-1-AP targets MIPEP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIPEP fusion protein Ag1451 相同性解析による交差性が予測される生物種
Full Name mitochondrial intermediate peptidase
Calculated molecular weight 81 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC009934
Gene Symbol MIPEP
Gene ID (NCBI) 4285
RRIDAB_2235137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MIPEP(Mitochondrial intermediate peptidase) is also named as MIP and belongs to the peptidase M3 family. It performs the final step in processing a specific class of nuclear-encoded proteins targeted to the mitochondrial matrix or inner membrane. The enzyme is a monomer of 75 kDa with a transit peptide and about 20% of the fmal MIP preparation consists of equimolar amounts of two peptides of 47 kDa and 28 kDa, which are apparently the products of a single cleavage of the 75 kDa protein. MIP is an independent endopeptidase acting on mitochondrial intermediate proteins as substrates.(PMID:1322290).

Protocols

Product Specific Protocols
WB protocol for MIPEP antibody 11002-1-APDownload protocol
IHC protocol for MIPEP antibody 11002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Clin Proteomics

ESF1 and MIPEP proteins promote estrogen receptor-positive breast cancer proliferation and are associated with patient prognosis

Authors - Qing Yu
View Article
humanWB

Biochem Biophys Res Commun

Mitochondrial intermediate peptidase: expression in Escherichia coli and improvement of its enzymatic activity detection with FRET substrates.

Authors - Marcondes Marcelo F MF
View Article