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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MIS12 Polyclonal antibody

MIS12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19820-1-AP

Synonyms

2510025F08Rik, hMis12, KNTC2AP, MIS12, MTW1, Protein MIS12 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MIS12 Polyclonal antibody (19820-1-AP)

    WB analysis using 19820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19820-1-AP (MIS12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19820-1-AP targets MIS12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIS12 fusion protein Ag13870 相同性解析による交差性が予測される生物種
Full Name MIS12, MIND kinetochore complex component, homolog (yeast)
Calculated molecular weight 205 aa, 24 kDa
Observed molecular weight 25-28 kDa
GenBank accession numberBC000229
Gene symbol MIS12
Gene ID (NCBI) 79003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The MIS12 complex, composed of the Mis12, Pmf1, Nsl1 and Dsn1 subunits, is a highly conserved kinetochore protein that interacts with the Ndc80 and Knl1 complexes, forming the KMN network (PMID: 32341029). MIS12 is a part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. MIS12 is essential for proper kinetochore microtubule attachments (PubMed:23891108)

Protocols

Product Specific Protocols
WB protocol for MIS12 antibody 19820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols