MITF Recombinant antibody
MITF Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
240815E7
Cat no : 83803-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | HeLa cells, HepG2 cells, A431 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83803-2-RR targets MITF in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MITF fusion protein Ag3679 相同性解析による交差性が予測される生物種 |
Full Name | microphthalmia-associated transcription factor |
Calculated molecular weight | 91 aa, 10 kDa, 59 kDa |
Observed molecular weight | 60-65 kDa |
GenBank accession number | BC012503 |
Gene symbol | MITF |
Gene ID (NCBI) | 4286 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases (PMID:22523078). Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells (PMID: 10578055).
Protocols
Product Specific Protocols | |
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IF protocol for MITF antibody 83803-2-RR | Download protocol |
FC protocol for MITF antibody 83803-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |