Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, rat pancreas tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 6 publications below |
Product Information
22772-1-AP targets MIXL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MIXL1 fusion protein Ag18732 相同性解析による交差性が予測される生物種 |
| Full Name | Mix1 homeobox-like 1 (Xenopus laevis) |
| Calculated molecular weight | 232 aa, 25 kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | BC113441 |
| Gene Symbol | MIXL1 |
| Gene ID (NCBI) | 83881 |
| RRID | AB_2879164 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q9H2W2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Mix1 homeobox-like 1 (MIXL1) Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression. This antibody is specific to MIXL1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MIXL1 antibody 22772-1-AP | Download protocol |
| IF protocol for MIXL1 antibody 22772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network. | ||
Cell Death Differ Uric acid: a potent molecular contributor to pluripotent stem cell cardiac differentiation via mesoderm specification. | ||
Front Cell Dev Biol Species origin of exogenous transcription factors affects the activation of endogenous pluripotency markers and signaling pathways of porcine induced pluripotent stem cells | ||
STAR Protoc Accelerated protocol for the differentiation of podocytes from human pluripotent stem cells. |