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- KD/KO Validated
MKL1 Polyclonal antibody
MKL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 21166-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human small intestine tissue, human tonsillitis tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 23 publications below |
| IHC | See 2 publications below |
| IF | See 14 publications below |
| ChIP | See 4 publications below |
Product Information
21166-1-AP targets MKL1 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MKL1 fusion protein Ag15240 相同性解析による交差性が予測される生物種 |
| Full Name | megakaryoblastic leukemia (translocation) 1 |
| Calculated molecular weight | 931 aa, 99 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC115039 |
| Gene symbol | MKL1 |
| Gene ID (NCBI) | 57591 |
| RRID | AB_2878822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MKL1 antibody 21166-1-AP | Download protocol |
| IHC protocol for MKL1 antibody 21166-1-AP | Download protocol |
| IF protocol for MKL1 antibody 21166-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling. | ||
Nat Cell Biol Myc-dependent dedifferentiation of Gata6+ epidermal cells resembles reversal of terminal differentiation | ||
J Cell Biol The actin-MRTF-SRF transcriptional circuit controls tubulin acetylation via α-TAT1 gene expression. | ||
Elife Differential regulation of cranial and cardiac neural crest by serum response factor and its cofactors. | ||
Exp Mol Med MRTF-A-NF-κB/p65 axis-mediated PDL1 transcription and expression contributes to immune evasion of non-small-cell lung cancer via TGF-β
| ||
Oncotarget The Wnt-β-catenin signaling regulated MRTF-A transcription to activate migration-related genes in human breast cancer cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Qingxue (Verified Customer) (06-20-2018) |
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