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Muskelin Polyclonal antibody
Muskelin Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 14735-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells, Jurkat cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
14735-1-AP targets Muskelin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Muskelin fusion protein Ag6147 相同性解析による交差性が予測される生物種 |
| Full Name | muskelin 1, intracellular mediator containing kelch motifs |
| Calculated molecular weight | 735 aa, 85 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC067825 |
| Gene symbol | Muskelin |
| Gene ID (NCBI) | 4289 |
| RRID | AB_2142504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Muskelin antibody 14735-1-AP | Download protocol |
| IHC protocol for Muskelin antibody 14735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Muskelin Coordinates PrPC Lysosome versus Exosome Targeting and Impacts Prion Disease Progression.
| ||
Sci Rep Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin. | ||
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH S (Verified Customer) (01-01-2022) | This antibody is ok, but not the best. At least, KO validation. The weak signal could be due to the low protein expression level though.
 |
FH H (Verified Customer) (04-05-2020) | Primary antibody: 1:1000 dilution in 5% BSA, over night at 4CSecondary antibody: Rabbit IRDye800Detection: odyssey ClxI was able to detect the band in HEK293T and HCT116 cells. However, the band intensity was not that strong.
 |
