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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MKNK2 Recombinant antibody

MKNK2 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240045B3

Cat no : 83235-1-RR

Synonyms

MAPK signal-integrating kinase 2, MAP kinase-interacting serine/threonine-protein kinase 2, MAP kinase signal-integrating kinase 2, GPRK7, EC:2.7.11.1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using MKNK2 Recombinant antibody (83235-1-RR)

    IF Staining of MCF-7 using 83235-1-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MKNK2 antibody (83235-1-RR, Clone: 240045B3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using MKNK2 Recombinant antibody (83235-1-RR)

    FC experiment of HeLa using 83235-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of MCF-7 cells using MKNK2 Recombinant antibody (83235-1-RR)

    FC experiment of MCF-7 using 83235-1-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MKNK2 Recombinant antibody (83235-1-RR, Clone:240045B3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83235-1-RR

    Affinity and Kinetic Characterization of 83235-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83235-1-RR against Human MKNK2 were performed. The affinity constant is 75.1 pM.

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83235-1-RR targets MKNK2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MKNK2 fusion protein Ag10546 相同性解析による交差性が予測される生物種
Full Name MAP kinase interacting serine/threonine kinase 2
Calculated molecular weight 465 aa, 52 kDa
Observed molecular weight 47-52 kDa
GenBank accession numberBC018345
Gene symbol MKNK2
Gene ID (NCBI) 2872
RRIDAB_3670912
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

11

Protocols

Product Specific Protocols
IF protocol for MKNK2 antibody 83235-1-RRDownload protocol
FC protocol for MKNK2 antibody 83235-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols