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- KD/KO Validated
MKP-2 Monoclonal antibody
MKP-2 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, rat, mouse
Applications
WB, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
1G1C12
Cat no : 66349-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, RAW 264.7 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
66349-1-Ig targets MKP-2 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, pig, rat, mouse samples.
| Tested Reactivity | human, pig, rat, mouse |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | MKP-2 fusion protein Ag1206 相同性解析による交差性が予測される生物種 |
| Full Name | dual specificity phosphatase 4 |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 43 kDa, 33 kDa |
| GenBank accession number | BC014565 |
| Gene symbol | DUSP4 |
| Gene ID (NCBI) | 1846 |
| RRID | AB_2881729 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DUSP4, also named as MKP-2, is a member of the mitogen-activated protein (MAP) kinase phosphatase family which has been suggested to play an important role in the feedback control of MAP kinase-mediated gene expression. DUSP4 preferentially inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. DUSP4 encodes two distinct isoforms: MKP-2-L(43 kDa) and MKP-2-S (33 kDa).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MKP-2 antibody 66349-1-Ig | Download protocol |
| IHC protocol for MKP-2 antibody 66349-1-Ig | Download protocol |
| IF protocol for MKP-2 antibody 66349-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration | ||
Cell Rep DUSP4 promotes esophageal squamous cell carcinoma progression by dephosphorylating HSP90β
| ||
Cancers (Basel) Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases | ||
Life Sci Alliance DOCK1 insufficiency disrupts trophoblast function and pregnancy outcomes via DUSP4-ERK pathway |
