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  • Featured Product
  • KD/KO Validated

MKRN2 Polyclonal antibody

MKRN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12238-1-AP

Synonyms

RING-type E3 ubiquitin transferase makorin-2, RING finger protein 62, makorin ring finger protein 2, HSPC070, EC:2.3.2.27

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MKRN2 Polyclonal antibody (12238-1-AP)

    WB analysis using 12238-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using MKRN2 Polyclonal antibody (12238-1-AP)

    WB analysis of K-562 using 12238-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using MKRN2 Polyclonal antibody (12238-1-AP)

    WB analysis of A375 using 12238-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12238-1-AP (MKRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MKRN2 Polyclonal antibody (12238-1-AP)

    IHC staining of human prostate cancer using 12238-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using MKRN2 Polyclonal antibody (12238-1-AP)

    IHC staining of human prostate cancer using 12238-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12238-1-AP (MKRN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MKRN2 Polyclonal antibody (12238-1-AP)

    IF Staining of U2OS using 12238-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MKRN2 Polyclonal antibody (12238-1-AP)

    IF Staining of U2OS using 12238-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MKRN2 antibody (12238-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, A375 cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12238-1-AP targets MKRN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MKRN2 fusion protein Ag2881 相同性解析による交差性が予測される生物種
Full Name makorin ring finger protein 2
Calculated molecular weight 416 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC015715
Gene symbol MKRN2
Gene ID (NCBI) 23609
RRIDAB_2142636
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MKRN2(Probable E3 ubiquitin-protein ligase makorin-2) is also named as RNF62. It is an E3 ubiquitin ligase that catalyzes the covalent attachment of ubiquitin moieties onto substrate proteins. It may be playing a functional role in negatively regulating neurogenesis via PI3K/Akt signaling.

Protocols

Product Specific Protocols
WB protocol for MKRN2 antibody 12238-1-APDownload protocol
IHC protocol for MKRN2 antibody 12238-1-APDownload protocol
IF protocol for MKRN2 antibody 12238-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.

Authors - Caiwei Jia
  • KD Validated
View Article
humanWB

Oncol Lett

Ubiquitination of P53 by E3 ligase MKRN2 promotes melanoma cell proliferation.

Authors - Yiling Zhang
  • KD Validated
View Article
humanWB

J Invest Dermatol

N6-methyladenosine modification of lnc-CCKAR-5 regulates autophagy in human umbilical cord mesenchymal stem cells by destabilizing LMNA and inhibits diabetic wound healing

Authors - Jian Wang
View Article