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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Melan-A Monoclonal antibody

Melan-A Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4F10D6

Cat no : 60348-1-Ig

Synonyms

Antigen LB39 AA, Antigen SK29 AA, MART 1, MART1, melan A, Melan-A , MLANA, Protein Melan A

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Melan-A Monoclonal antibody (60348-1-Ig)

    IHC staining of human malignant melanoma using 60348-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Melan-A Monoclonal antibody (60348-1-Ig)

    IHC staining of human malignant melanoma using 60348-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using Melan-A Monoclonal antibody (60348-1-Ig)

    IHC staining of human lung using 60348-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using Melan-A Monoclonal antibody (60348-1-Ig)

    IHC staining of human lung using 60348-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 60348-1-Ig (Melan-A antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Melan-A Monoclonal antibody (60348-1-Ig)

    IHC staining of human malignant melanoma using 60348-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Melan-A Monoclonal antibody (60348-1-Ig)

    IHC staining of human malignant melanoma using 60348-1-Ig

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 60348-1-Ig (MLANA Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive IHC detected inhuman malignant melanoma tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

60348-1-Ig targets Melan-A in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Melan-A fusion protein Ag13387 相同性解析による交差性が予測される生物種
Full Name melan-A
Calculated molecular weight 13 kDa
GenBank accession numberBC014423
Gene symbol MelanA
Gene ID (NCBI) 2315
RRIDAB_2881457
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Melan-A is a melanocyte differentiation antigen, recognized by autologous cytotoxic T lymphocytes. Melan-A is also called MART-1 (melanoma antigen recognized by T cells). The Melan-A/MART-1 gene encodes this protein, 20-22 kDa, associated with endoplasmic reticulum and melanosomes. The function of the protein is unknown. Melan-A, isolated as a melanoma-specific antigen, is a transmembrane protein, which is expressed in skin, retina and the majority of cultured melanocytes as well as in melanomas. Melan A is expressed in more than 85% of melanomas.

Protocols

Product Specific Protocols
IHC protocol for Melan-A antibody 60348-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancer Commun (Lond)

Candidate therapeutic agents in a newly established triple wild-type mucosal melanoma cell line.

Authors - Chaoji Shi
View Article