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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

MLF1 Polyclonal antibody

MLF1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13100-1-AP

Synonyms

myeloid leukemia factor 1, Myelodysplasia-myeloid leukemia factor 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse heart tissue using MLF1 Polyclonal antibody (13100-1-AP)

    WB analysis of mouse heart using 13100-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using MLF1 Polyclonal antibody (13100-1-AP)

    WB analysis of human brain using 13100-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using MLF1 Polyclonal antibody (13100-1-AP)

    WB analysis of mouse testis using 13100-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 13100-1-AP (MLF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using MLF1 Polyclonal antibody (13100-1-AP)

    IP experiment of mouse heart using 13100-1-AP

    IP result of anti-MLF1 (IP:13100-1-AP, 4ug; Detection:13100-1-AP 1:500) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLF1 Polyclonal antibody (13100-1-AP)

    IHC staining of human colon cancer using 13100-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using MLF1 Polyclonal antibody (13100-1-AP)

    IHC staining of human colon cancer using 13100-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13100-1-AP (MLF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse heart tissue, human brain tissue, mouse testis tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13100-1-AP targets MLF1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MLF1 fusion protein Ag3727 相同性解析による交差性が予測される生物種
Full Name myeloid leukemia factor 1
Calculated molecular weight 268 aa, 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberBC007045
Gene symbol MLF1
Gene ID (NCBI) 4291
RRIDAB_2147670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MLF1 antibody 13100-1-APDownload protocol
IHC protocol for MLF1 antibody 13100-1-APDownload protocol
IP protocol for MLF1 antibody 13100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs).

Authors - Daoyuan Huang
View Article